Until very recently five tribes have been recognized in the Mimosoideae, based primarily on combinations of a few characters: number and fusion of stamens and aestivation and fusion of the sepals. The Parkieae, Mimozygantheae, and Mimoseae were distinguished as having 10 stamens (considered plesiomorphic in the Mimosoideae): the Parkieae with imbricate aestivation of the fused calyx lobes; the Mimozygantheae with free, imbricate sepals; and the Mimoseae with the sepals valvate. The Acacieae and Ingeae, also with valvate aestivation of sepals, have either many free (Acacieae) or many fused (Ingeae) stamens. That these groups might not be natural has long been recognized, but improved classifications are only now beginning to emerge. This study combines data from several independent molecular studies of the tribes to improve our understanding of the relationships among mimosoid genera. Over 140 taxa were sampled. The chloroplast genes trnL and trnK/matK served as data sources for phylogeny reconstruction. Parsimony analysis of the data resulted in 11,326 most parsimonious trees. None of the tribes are monophyletic on the strict consensus tree. The Mimoseae are paraphyletic, with members of all other tribes derived from within it. The Parkieae are polyphyletic, with Parkia nested among the Piptadenia group in the tribe Mimoseae. The Acacieae and Ingeae are polyphyletic and form two clades. Acacia subg. Acacia is strongly supported as monophyletic, and is not closely related either to other species of Acacia s.lat. or the Ingeae. The remainder of the Acacieae and Ingeae form a monophyletic group, with the Australian acacias (subg. Phyllodineae) also strongly supported as a monophyletic group. Acacia subg. Aculeiferum is paraphyletic. Relationships among the Ingeae are poorly resolved and not well supported. This study highlights the inevitability of recognizing segregate genera from Acacia s. lat., and the necessary abandonment of Bentham's longstanding tribal classification.

Key words: Acacia, Leguminosae, MImosoideae, phylogeny