The Gloxinieae (Gesneriaceae) tribe includes 22 genera and approximately 290 species, as currently circumscribed. Recent molecular phylogenetic studies have suggested that some members of the tribe (the genera Sinningia, Paliavana, and Vanhouttea) are not closely related to the rest of the tribe. Additionally, other species (i.e., Gloxinia sarmentiana) appear to not belong to the Gloxinieae. The Gloxinieae have traditionally been troublesome, both in circumscription of generic boundaries and membership in tribal classifications. This is in part due to extreme variation in floral form in this group. In order to address questions of generic boundaries, tribal classification, the evolution of floral form, and biogeographic distribution patterns, we have analyzed morphological cladistic, nuclear ribosomal ITS sequence, and chloroplast DNA trnL-F spacer sequence data sets with maximum parsimony and maximum likelihood. These data sets support a number of findings. These include recognition of the genus Gloxinia, as currently circumscribed, as polyphyletic, that the genus Capanea is derived from within Kohleria, and that the tribe may have originated in Central America and the Caribbean, with later dispersal to South America.

Key words: classification, floral evolution, Gesneriaceae, Gloxinieae, molecular phylogenetics, morphological cladistics