Sequence variation in the internal and external transcribed spacers of nuclear ribosomal DNA allows the first analysis of phylogenetic relationships in the Melastomataceae subfamily Memecyloideae, a group of shrubs and small trees with about 450 currently recognized species and a pan-tropical distribution pattern. Earlier proposals to recognize four paleotropical genera (Memecylon, Spathandra, Lijndenia, and Warneckea) based on morphological and anatomical evidence are strongly supported by this molecular data set. Four east African taxa that had been transferred to Lijndenia are shown to belong either to Memecylon (M. fragrans, M. greenwayi, M. semsei) or to Warneckea (W. jasminoides). Within the large and widespread Old World genus Memecylon, the west African taxon M. lateriflorum is strongly supported as sister to the remaining taxa, corroborating the earlier-hypothesized trend toward decreasing locule number in this group (ovaries 4-loculed in members of sect. Mouririoidea versus unilocular in all other species of Memecylon). Species groups in Memecylon from east Africa, Madagascar, and Malesia are each well supported as monophyletic, and the low sequence variation observed within the Malagasy clade suggests that the high species diversity found on that island is the result of a recent radiation.

Key words: ETS, ITS, Melastomataceae, Memecyloideae, molecular phylogeny, rDNA