Zaluzianskya (Scrophulariaceae, Tribe Manuleae) comprises 54 species of annual or perennial herbs distributed throughout South Africa and displaying diverse floral morphology. Preliminary molecular phylogenetic analyses, based on both nuclear and cpDNA sequences, will be discussed for Zaluzianskya. These estimates of phylogeny will then be used to investigate the evolution of floral characteristics within this genus. Although increased taxon sampling is necessary, several tentative conclusions can be drawn. Two of the four sections of this genus are clearly not monophyletic: sections Zaluzianskya and Holomeria. The status of sections Nycterinia and Macrocalyx is uncertain. Day flowering is ancestral within Zaluzianskya, with night flowering evolving at least twice along with at least one reversal to day flowering.

Key words: character evolution, Scrophulariaceae, South Africa, systematics, Zaluzianskya