Variations in floral development are of major evolutionary and economic importance, impacting features such as pollination, fruit development, and seed dispersal. However, despite the central role of flowers in plant reproduction and agriculture, the processes responsible for the origin and evolution of the flower remain central problems in plant biology. Major questions include: how did perianth organs (sepals and petals) and carpels originate? How did flowers become bisexual? What causes variation in sepal, petal, stamen, and carpel structure? What regulatory pathways control floral development throughout the angiosperms, and how do they compare with those of model plants? The newly established Floral Genome Project (FGP) is a multi-university project that will address these and other questions about the origin and diversification of flowers and the floral developmental program? The project will involve large-scale EST and cDNA squencing of 15 plant species, followed by microarray and in situ expression studies, and extensive bioinformatic and molecular evolutionary analysis. Many new resources will be generated for the botanical community, including clones and sequences of a large number of plant nuclear genes in diverse basal angiosperms, monocots, eudicots, and gymnosperms. This talk will introduce the goals and methods of the FGP, and provide a progress report on the initial steps of taxon selection, tissue collection and RNA isolation, library building, and EST sequencing.

Key words: Acorus, Amborella, Escholtzia, Flower development, Nuphar, Welwitschia