Phenetic variation and relationships between the Vigna angulais complex including wild (V. angularis var. nipponensis), weedy (V. angularis), and cultivated types (V. angularis var. angularis), V. nakashimae, and V. umbellata from Korea were examined using AFLP methodology. AFLP analysis of 50 accessions revealed 71.1% polymorphic fragments out of 462 fragments amplified using seven primer combinations. The polymorphic fragment proportion was the highest in P32/ M34 (86.8%), while the lowest in P33/ M34 (57.1%). The number of polymorphic fragment of each species was 70 in the V. angulairs complex and 41 in V. nakashimae, but there was no polymorphism in rice bean (V. umbellata). The number of shared fragments among species ranges from 142 between the V. angularis complex and V. nakashimae to 166 between the V. angularis complex and V. umbellata. On the other hand, its ranges were from 231 between cultivated and weedy types and to 238 between cultivated and wild types within V. angularis complex. A dendrogram generated from Jaccard¡¯s similarity matrix was divided into three groups, which correspond to V. nakashimae, V. angularis complex, and V. umbellata. The V. angularis complex is more closely related to V. umbellata than V. nakashimae. Phenetic distances were average of 0.502 between the V. angularis complex and V. nakashimae and 0.467 between the V. angularis complex and V. umbellata. Within the V. angularis complex, the weedy type was more related with wild than cultivated types. But a two dimensional PCA plot of the V. angularis complex, which accounts for 47.4% of variation, reveals that each types are not clearly separated. These results will help to understand genetic diversity and evolutionary dynamics of Vigna and to broaden the germplasm.

Key words: AFLP, azuki bean complex, Ceratotropis, phenetic distance, relationship, Vigna angulairs