Neotropical flowers exhibit a large diversity in shape, size, and color. To understand the evolution of the complexity of floral traits, these characters need to be evaluated in the context and constraints of detailed phylogenetic data. Hypotheses of time and place of the origin of floral characters are only well-founded when supported by detailed morphological and anatomical investigations as well as robust phylogenetic data (morphological and/or molecular). For example, the monophyletic tribe Helieae (Gentianaceae; 22 genera, ca. 190 spp.) includes 0.5-15 cm long flowers that are pollinated by flies, bees, hawk-moths, bats, or hummingbirds. An array of floral characters is represented, but no previous work has attempted to present evolutionary hypotheses for these. The flowers have the sympetalous bauplan characteristic of hypogynous Asteridae, but are uncommon in having zygomorphic stamens and styles. The anthers are usually strongly bent after anthesis and positioned in or protruding from the lower part of the corolla mouth. Such a placement of the anthers might facilitate increased pollination by larger animals such as bats or hummingbirds, which is also supported by our phylogenetic trees based on ITS sequence data. Large, red-pink(-yellow) flowers pollinated by hummingbirds is a derived trait, most likely derived from bat-pollinated flowers. Symbolanthus has a ring-shaped corona inside the corolla at the base of the stamens. New investigations show that coronas are strongly variable in size and shape between different species. Staminal gaps between the corolla tube and the staminal base are found in Symbolanthus and close relatives and may have been a precursor to the evolution of the corona. The unique valvate-contort corolla aestivation of the presumably hawk-moth-pollinated Aripuana is also discussed. A hypothesis for the development of and homology among corolla parts in contort (stamens in corolla tube) vs. valvate-contort (stamens in sinuses) corolla buds is presented.

Key words: Asteridae, floral anatomy, floral evolution, Gentianaceae, molecular systematics, pollination