POLANS, NEIL O.*, JEFFERY A. NELSON, DANA R. KURPIUS, and DAYLE E. SAAR. Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL. 60115. - A phylogenetic study of wild species and selected cultivars of pea.
Wild populations and selected cultivated accessions of the genus
Pisum are evaluated for morphological characters, allozymes,
RAPDs, ISSRs and ITS sequences. Tests of skewness and partition
homogeneity indicate that each of these individual data sets exhibits
strong phylogenetic signal (g1 values ranging from -0.631 to -0.755,
P<<0.01), and sufficient congruence (P values ranging from 0.516 to
1.000) to support their combination in a single data set. Even the
small number of morphological characters generally organizes the
species into traditional taxonomic groupings using both maximum
parsimony and bootstrap methods. Individual and combined molecular
data sets support several of these same associations. Decay indices
and other branch values for the comparatively data-rich ISSR and RAPD
trees reveal strong support for three nodes that: 1) separate P.
fulvum from the P. humile, P. elatius and P. sativum
ingroup; 2) establish the northern humile, elatius and
sativum clade; and 3) define sativum as a monophyletic
group. The southern humile populations form a clade that is
distinct from fulvum and also from elatius, sativum and
even northern humile in the combined-data tree; although, the
southern humile node is supported only tenuously by the
individual molecular trees. The RAPD trees suggest that elatius
may be the sister taxon to the cultivated sativum, a
relationship that is observed in the combined-data tree. By
comparison, the ISSR trees present only modest support for northern
humile as the closest relative of the domesticated pea. A small
number of polymorphic ITS sites places northern humile further
from sativum and emphasizes the close affinity among all the
non-fulvum peas. Species fulvum is clearly the most
distinct of the pea taxa in every analysis.
Key words: allozymes, ISSRs, ITS, phylogenetics, Pisum, RAPDs