HARGROVE, LORI and MICHAEL G. SIMPSON.* Department of Biology, San Diego State University, San Diego, California 92182, U.S.A. - Ultrastructure of heterocolpate pollen in Cryptantha (Boraginaceae).
Pollen of eight species of Cryptantha was studied using
scanning electron microscopy. In addition, transmission electron
microscopy was used in Cryptantha intermedia to elucidate the
ultrastructural basis for their heterocolpate pollen, in which three
"true" colporate apertures alternate with three
"pseudoapertures." Both apertures and pseudoapertures are
regions of the wall in which ektexine is largely absent. However,
apertures have an outer band of verrucate exinous deposits, a band of
inner intine wall material, and a concentration of cytoplasmic
vesicles. In addition, apertures are shorter in length and wider at
the equator than are pseudoapertures. The exine wall structure in
Cryptantha intermedia consists of a typical inner, homogeneous
endexine (thickened at both apertures and pseudoapertures) and, in
non-apertural regions, an outer, essentially imperforate,
tectate-columellate ektexine. Curious triangular "polar
pseudoapertures," devoid of ektexine, occur at each pole and
resemble the pseudoapertures in sculpturing and wall structure. The
described heterocolpate pollen in the Boraginaceae may represent a
major apomorphy for the tribes Eritricheae, Cynoglosseae, and possibly
other taxa. However, further comparative studies within these groups
are needed to definitively assess the extent of this putative
apomorphy and to identify and characterize palynological features that
may be useful within these groups.
Key words: Boraginaceae, Cryptantha, pollen, ultrastructure