Phylogenetic relationships within Gomphrenoideae (Amaranthaceae) remain unresolved. The subfamily contains approximately 14 genera and 374 species; all of the genera occur in America with some species distributed in Africa, Australia, and Japan. Within Gomphrenoideae, the classification of the North American genus Tidestromia has been controversial. To test the monophyly of Tidestromia and to determine the phylogenetic relationships among the species, six recognized species were included in a cladistic analysis using Alternanthera sessilis, A. pycnantha, Gossypianthus lanuginosus, Guilleminea densa, and Froelichia interrupta as outgroups. In total, 15 micro and macro-morphological characters were scored. They were observed from vegetative and reproductive structures studied in herbarium material and through the use of scanning electron microscopy. Phylogenetic analysis of the morphological data supported Tidestromia as monophyletic. This clade is supported by tepals with ramified trichomes and dichasium inflorescences. Although resolution among the species of Tidestromia was poor, Tidestromia lanuginosa, T. carnosa, and T. tenella form a monophyletic clade supported by the annual habit. The analysis yielded 18 most-parsimonious trees of 26 steps, with a consistency index (CI) of 0.80 and retention index (RI) of 0.84. A combined data analysis using sequences of the 5S non-transcribed spacer and the nuclear ribosomal ITS region will also be included in this investigation.

Key words: Amaranthaceae, Froelichiinae, Gomphreneae, Gomphrenoideae, North America., Tidestromia