RIDING, R.T.1*, M.B. LAVIGNE2, and C.A. LITTLE2. 1Biology Department, University of New Brunswick, P.O. Bag Service 45111, Fredericton, New Brunswick E3B 6E1, Canada; 2Natural Resources Canada, Canadian Forest Service, Atlantic Forestry Centre, P.O. Box 4000, Fredericton, New Brunswick E3B 5P7, Canada. - Stem respiration rate: a non-destructive marker of xylem cell
production.
In the year 2000 we set out to document the relationship between
respiration and cambial activity in a Gymnosperm, balsam fir (Abies
balsamea), a ring-porous Angiosperm, white ash (Fraxinus
americana) and and a diffuse-porous Angiosperm red maple (Acer
rubrum). To this end two sets of neighbouring trees were selected
for each species. One set was fitted with respiration chambers and
respiration was measured three times per week throughout the
experiment from March through July. The other set had wood samples
extracted at three times, 21 March, 4 May, and 6 June, for anatomical
study. On the final sampling date, 21 July, samples were taken from
stems beneath the centre of the respiration chambers for microscopic
examination. We found that respiration did increase during cambial
reactivation. This occurred first in balsam fir followed by ash, both
species had new xylem and phloem in the May samples, and finally by
red maple. In the maple there was evidence of new phloem production in
the May sample, however, the increase in respiration and xylem cell
production occurred starting after the June wood sample date. Thus, it
appears that respiration rate is a good marker for xylem cell
production. Moreover, rate of respiration at the peak of the growing
season, on 21 July, was correlated to the number of tracheids or
fibres produced by the cambium up to that point in the growing season,
and this correlation is across the three species.
Key words: Abies balsamea, Acer rubrum, cambium, Fraxinus americana, respiration, xylem