RICHARDSON, CHARLES RODGERS*, MIKE BOWLIN, and D. NICHOLAS MC LETCHIE. T.H. Morgan School of Biology, University of Kentucky, Lexington Ky, 40502. - Differences in early life-history traits of two propagule types in the liverwort Marchantia inflexa.
Marchantia inflexa is a dioecious, thallose bryophyte that exhibits
both sexual and asexual modes of reproduction. Sexual reproduction
results in the production of spores, while asexual reproduction
results in the production of asexual propagules, gemmae. This
experiment compared the differences in early life history of spores
and gemma under two different light treatments. Spores were collected
from 4 mothers that were fertilized with up to 4 fathers and
germinated in either a 45% or 37% light environment. Upon germination,
100 sporelings from each light treatment were transferred to pots
filled with steamed soil and placed in the same light conditions as
they were germinated. Simultaneously, 6-8 male and female genotypes
were randomly chosen to collect gemmae to make sex-specific mixtures
of gemmae. One hundred male and one hundred female gemmae were
individually planted and evenly divided among the light treatments.
Thrice weekly we recorded the life-history characters of time to first
meristem split and first cup production. In bi-weekly censuses, we
recorded cup number and photographs were taken to assess size. A
repeated measures analysis of plant size revealed that the pattern of
growth was affected by propagule type, light and the interaction of
propagules and light. Gemmae plants were significantly larger at early
dates, but there was no significant difference in size by the final
census. Similarly, a MANOVA revealed significant effects of propagule
type, light and the interaction of propagules and light on
life-history traits on the final census date. Gemmae plants reached
first meristem split earlier than spore plants, but spore plants began
producing cups earlier and had more cups per unit area than gemma
plants. These experimental results suggest that environmental
conditions could influence the relative success of sexual and asexual
reproduction under field conditions.
Key words: asexual reproduction, bryophyte, Life-history, Marchantia inflexa