INGRAM, AMANDA L.* and JEFF J. DOYLE. L. H. Bailey Hortorium, 462 Mann Library, Cornell University, Ithaca, NY 14853. - Sprint: A new family of MITEs (miniature inverted-repeat elements) in Uniola paniculata L.
MITEs (miniature inverted-repeat transposable elements) are a class of
transposable element first described from an insertion in the
waxy gene of maize (Zea mays) that are found in the
genomes of a wide range of eukaryotes. MITEs are short AT-rich
elements that are found in high copy number in the genome, have a
characteristic hairpin secondary structure, and are bounded by a short
terminal inverted repeat (TIR). We have discovered a putative MITE
inserted in the ninth intron of the waxy gene (granule-bound
starch synthase I; GBSSI) in Uniola paniculata L., a species in
subfamily Chloridoideae of the Poaceae. MITEs are known from a wide
range of grasses, but this is the first such element described in this
subfamily. The element is 212 bp long, has high AT content (85%), has
a 10-bp TIR, and exhibits the hairpin secondary structure typical of
MITEs. This element has no sequence similarity to previously described
MITEs and has a unique insertion site sequence. These qualities
indicate that this element belongs to a new family of transposable
elements, which we have called Sprint. Properties of the
element including copy number and taxonomic distribution will be
discussed.
Key words: Chloridoideae, MITE, Poaceae, transposable element, Uniola paniculata