FISHMAN, LILA1*, CHERYL L. GONZALES2, and JOHN H. WILLIS1. 1Dept. of Biology, Duke University, Durham, NC 27708; 2Dept. of Biology, University of Oregon, Eugene, OR 97403. - Natural selection on flower size in Mimulus guttatus?
Genetic variation within populations provides the raw material for
adaptive divergence between populations and species, and understanding
the fitness consequences of such variation is a major goal of
evolutionary biology. Here, we explore correlations among floral
traits, individual selfing rates and female fitness within a wild
population of the primarily outcrossing plant Mimulus guttatus.
Previous work has shown that floral variation in this population is
highly heritable and not primarily due to deleterious mutations at low
frequency. Small flowers and high selfing rates have evolved several
times in closely related members of the M. guttatus species
complex, potentially to assure reproduction under conditions of pollen
limitation. On the other hand, phenotypic measures of selection
typically find positive relationships between floral size characters
and fitness. In this study, we used a combination of experimental
manipulations and molecular marker-based paternity analysis to tease
apart the complex functional relationships among floral characters,
self-fertilization and fitness in the field. We address the following
questions: Is there pollen limitation of female fitness and, if so,
does it vary with floral morphology? Is individual variation in floral
traits (corolla size, stigma-anther separation) correlated with
selfing rate? With fitness? What ecological or genetic factors
generate such correlations? These results complement our growing
understanding of the genetic basis of mating system divergence within
the Mimulus guttatus complex.
Key words: floral variation, Mimulus guttatus, phenotypic selection, selfing rate