DEPAMPHILIS, CLAUDE1*, LENA LANDHERR1, TODD BARKMAN1,2, JOEL MCNEAL1, and NED YOUNG3. 1Department of Biology and Institute of Molecular Evolutionary Genetics, Penn State University, University Park, PA 16801; 2Department of Biology, Western Michigan University, Kalamazoo, MI 49008; 3Biology Department, Trinity University, San Antonio, TX 78212. - Riveting reverses editing in plant mitochondrial sequences.
RNA editing is a post transcriptional process that alters the sequence
of individual RNAs prior to translation. In plants, C to U editing is
common in both mitochondrial and chloroplast sequences, where editing
can create altered start or stop codons and often results in proteins
whose sequence is different than that predicted by the DNA sequence
alone. Editing in plants is widely interpreted as a correction
mechanism for T to C mutations, and may have additional significance
in regulation of gene and protein function. We have experimentally
determined RNA editing sites for the cox1 gene from 18 species of
angiosperms and inferred the status of editing sites for over 200
additional species. 1) Editing status for editable sites can be
accurately inferred for cox1 gene from a careful inspection of DNA
sequences, 2) Phylogenetic analysis of cox1 sequences is strongly
affected by the inclusion of these sites. When editing sites are
removed, phylogenies are consistent with hypotheses inferred from
chloroplast and/or nuclear genes. When editing sites are included in
the analysis, the effects depend on the choice of taxa but in some
cases phylogenies are serious disrupted. 3) Abrupt reversals of the
editing status ("Riveting") of all edited sites to create
"processed paralogs" (sensu Bowe and dePamphilis, 1996) has
occurred at least a dozen times during flowering plant evolution and
reversal of shorter contiguous regions of sequence have occurred at
least as frequently. These observations shed light on recombination
and reverse transcription mediated events in the plant mitochondrion.
Key words: coxI, mitochondrial DNA, phylogeny, processed paralog, RNA editing