Sibara filifolia (Brassicaceae) is a diminutive annual that was presumed extinct until it was rediscovered on San Clemente Island in 1986. It is known from populations on three adjacent ridges within 150m of each other, and from occasional scattered individuals nearby. Electrophoretic data for 29 allozyme loci indicate low overall levels of genetic variation, with only two polymorphic loci and low heterozygosity (HE=0.009). However, all polymorphism occurred in just one of the three populations, resulting in significant differentiation among populations (FST=0.145). Surveys of genetic variation using more than 20 polymorphic RAPD markers revealed a similar level of differentiation among populations (FST=0.159). The relatively high differentiation among populations, despite their close proximity, may be due to inbreeding, limited seed dispersal, and small population size.

Key words: allozymes, conservation, endemic, genetic differentiation, genetic diversity, RAPDs