An intraspecific phylogenetic study was undertaken to resolve the evolutionary relationship of isolates of the green alga Phyllodictyon anastomosans (Harv.) Kraft et Wynne that occur on Atlantic and Pacific coasts of the Central American Isthmus. Patterns of vicariance related to the emergence of the Central American Isthmus were evident, but numerous examples of recent trans-oceanic and trans-isthmian dispersal obscured the underlying pattern. This study, one of the first studies to assess the impact of the emergence of the Central American Isthmus in seaweed phylogeography, provided an ideal opportunity to estimate the rate of sequence evolution using a single time point. Using this newly calibrated molecular clock the timing of an historical introduction across the isthmus is shown to be concordant with a shallow water breach of the isthmus dated at approximately 2.3-2.0 million years ago. This work contributes to a growing body of literature that suggests marine algae are fairly successful at dispersing over long distances in recent times.

Key words: Central American Isthmus, molecular clock, Phyllodictyon anastomosans, phylogeography, rDNA ITS, vicariance