WATANABE, S.1*, K. MITUI2, T. NAKAYAMA2, and I. INOUYE2. 1Department of Education, Toyama University, Toyama, 930-8555, Japan; 2Institute of Biological Sciences, University of Tsukuba, Ibaraki 305, Japan. - Phylogenetic analyses of the species of Chlorosarcinopsis and Neochlorosarcina (Chlorophyceae).
The genus Chlorosarcinopsis forms sarcinoid packages of @cells
and reproduces by forming naked biflagellate zoospores. The genus
Neochlorosarcina is distinguished from Chlorosarcinopsis
in having a thin cell wall around zoospores. We analyzed phylogenetic
relationships of seven species of Chlorosarcinopsis and four
species of Neochlorosarcina using 18SrDNA sequence data. The
species of the Chlorosarcinopsis were separated into two
clades, and those of Neochlorosarcina formed a monophyletic
branch, which was far from the Chlorosarcinopsis clades.
Groover and Bold (1969) investigated physiological abilities of
species of Chlorosarcinopsis (which included species of
Neochlorosarcina), and showed that the species were roughly
divided into three groups by usage of nitrogen and carbon components.
These groups correspond two clades of Chlorosarcinopsis and a
clade of Neochlorosarcina. This result gave a phylogenetic
meaning to the differences in the physiological abilities, and
supported the separation of Neochlorosarcina from
Chlorosarcinopsis based on a morphological attribute.
Key words: Chlorophyceae, Chlorosarcinopsis, green algae, Neochlorosarcina, phylogeny