FAY, MICHAEL F.1*, ROBYN S. COWAN1, and TIM C. G. RICH2. 1Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3DS, UK; 2National Museum and Gallery, Cardiff, CF10 3NP, UK. - Patterns of genetic variation in the Sorbus latifolia aggregate (Rosaceae) - evidence from nuclear AFLP and plastid DNA sequences.
The Sorbus latifolia aggregate includes a group of mostly
apomictic taxa thought to result from hybridization between S.
torminalis and members of the S. aria aggregate. Some of
these taxa are widespread, but others are local endemics, occuring in
'diversity hotspots', including the Wye Valley and the Avon Gorge in
the UK. Nearly all of these endemics are extremely rare and protected
by national legislation. Amplified fragment length polymorphism (AFLP)
fingerprints demonstrate clear additivity in the S. latifolia
aggregate between 'aria' and 'torminalis' fingerprints.
One member of the S. aria aggregate, S. rupicola, has
been suggested to be a parent of some members of the S.
latifolia aggregate, although it can itself be diploid, triploid,
or tetraploid and thought to result from hybridization between S.
aria and another species, so far unidentified. On the basis of
AFLP, we have been able to exclude S. rupicola in one case.
Plastid DNA sequences for the trnL intron and the
trnL-trnF intergenic spacer for members of the S.
latifolia aggregate tested so far match the sequence for S.
torminalis, indicating that for these samples, at least, the
direction of hybridization is the same, with S. torminalis
being the plastid donor (presumably female) parent.
Key words: AFLP, apomixis, hybridization, plastid DNA, Sorbus