The monophyletic clade comprising Myrtaceae, Heteropyxidaceae, Psiloxylaceae, and Vochysiaceae represents a remarkable example of tropical / southern temperate disjunctions. Myrtaceae have centers of diversity in Australasia and South America, but little representation in Africa; the three species of Heteropyxidaceae are African; the single species of Psiloxylaceae is confined to the Mascarenes; and Vochysiaceae are largely tropical American but with two genera and three species restricted to west tropical Africa. A molecular phylogenetic analysis (rbcL, ndhF, matK) of the order Myrtales and with emphasis on this Myrtaceae clade and its sister group (Melastomataceae, Memecylaceae, Penaeaceae, Rhynchocalycaceae, Oliniaceae, Alzateaceae, Crypteroniaceae) was conducted under maximum parsimony and maximum likelihood. Vochysiaceae are sister to Myrtaceae, Heteropyxidaceae, and Psiloxylaceae, with the latter two strongly monophyletic. The neotropical Myrtaceae (largely comprising the subf. Myrtoideae) are well imbedded within a paraphyletic, capsular-fruited, largely Old World subf. Leptospermoideae. Sequence evolution in the Myrtaceae clade is significantly slower than in Melastomataceae and somewhat slower than in the Crypteroniaceae clade — illustrating generation time effects. Various molecular dating approaches were used to infer ages of specific nodes within the Myrtaceae clade of four families using calibrated fossil dates from Myrtaceae, Melastomataceae, Onagraceae, and Combretaceae. The results argue for a Gondwanan origin of the common ancestor of Myrtaceae, Heteropyxidaceae, Psiloxylaceae, and Vochysiaceae. Within Myrtaceae, the derivation of the several neotropical and African lineages may well be due to more recent dispersal. However, the timing of these events is somewhat obscured by the weak support for the backbone in the cladogram of Myrtaceae. With the amphi-Atlantic distributed Vochysiaceae now being placed as sister to the other three families, a vicariance explanation for its distribution can not be ruled out with the taxon sampling to date.

Key words: dispersal, Heteropyxidaceae, Myrtaceae, Psiloxylaceae, vicariance, Vochysiaceae