PETERSON, R. LARRY. Department of Botany, University of Guelph, Guelph, Ontario, Canada N1G 2W1. - Cytological characterization of Arum and Paris-type arbuscular mycorrhizas.
Two major types of arbuscular mycorrhizal associations, the
Arum-type and the Paris-type were recognized by Gallaud
in 1905 based on structural features of the colonization process.
Although the Paris-type is the most common (Smith and Smith,
1997), the majority of research concerning structure/function of
arbuscular mycorrhizas has involved the Arum-type because of
its prevalence among important crop species. Recently, we have studied
the Paris-type arbuscular mycorrhiza association in Panax
quinquefolius (ginseng). In particular, we have examined the
interface between the cytoplasm of root cells and the extensive hyphal
coils and the arbuscules that develop from them (arbusculate coils)
using various affinity methods combined with TEM. Also, we have
determined the effect of hyphal coils and arbusculate coils on the
cytoskeleton of ginseng root cells using immunofluorescence techniques
combined with laser scanning confocal microscopy. The results of these
studies will be compared with published work by ourselves and others
(particularly research by P. Bonfante and M. Harrison) on the
interface and changes in cytoskeleton in Arum-type mycorrhizas.
The structural features will be discussed in terms of functioning of
these two major types of arbuscular mycorrhizas.
Key words: arbuscular mycorrhiza, Arum-type, cytoskeleton, interface, Paris-type, structure