Bloom-forming dinoflagellates often synthesize high concentrations of mycosporine-like amino acids (MAAs) as a protection from high PAR and UV radiation, which may afford them a competitive advantage over other species. Photoprotection and long-term acclimation to natural and enhanced UVB radiation were examined during 4 weeks in three dinoflagellates and one diatom. Semi-continuous, nutrient-replete cultures were maintained outdoors under natural light, with or without additional UVB (from lamps). The photosynthetic performance (XE-PAM fluorometry) and the concentration and composition of MAAs and photoprotective carotenoids (PPCs; comprising xanthophylls) were followed weekly. A great variety of MAAs was found in the dinoflagellates (up to 10, identified by co-chromatography and LC-MS). Large differences between sp. (and between 2 strains of the same sp.) were observed. In most cases, the composition and concentration of MAAs and PPCs increased under enhanced UVB. Growth rate and photosynthetic performance were sometime lower under enhanced UVB, while in other cases photoacclimation seemed to occur. The least affected sp. was Alexandrium tamarense who responded most rapidly to enhanced UVB. The algae also responded to changes in natural incident radiation. Some methodological aspects of MAA quantification were also investigated (with 4 sp. of dinoflagellates). Our results indicate how freezing (liquid-N) likely breaks the cells, mobilizing the water-soluble MAAs in the filter moist (otherwise highly packaged in fresh cells), while the organelles containing the pigments remain relatively intact. The implications of standard sample handling on the evaluation of MAA concentration in algae and on the estimation of the absorption of suspended material in water from in vivo spectroscopy (filter pad technique) will also be discussed.

Key words: Alexandrium tamarense, in vivo absorption, MAAs, methodology, photoprotective carotenoids, phytoplankton